Fluorescence Multi-Detection Device #VitaiStream #Labtech_Holter #Pedcath8 #Arteriograph24 #Technosensor

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array by Victor Moro 1,*ORCID,Joan Canals 1ORCID,Sergio Moreno 1ORCID,Steffen Higgins-Wood 2ORCID, Oscar Alonso 1,Andreas Waag 2,J. Daniel Prades 1,2 andAngel Dieguez 1,*ORCID 1 Electronic and Biomedical Engineering Department, University of Barcelona, 08028 Barcelona, Spain 2 Institute of Semiconductor Technology, Technical University of Braunschweig, 38106 Braunschweig, Germany * Authors to whom correspondence should be addressed. Biosensors 2024, 14(6), 264; https://doi.org/10.3390/bios14060264 Submission received: 25 March 2024 / Revised: 29 April 2024 / Accepted: 21 May 2024 / Published: 22 May 2024 (This article belongs to the Special Issue Advanced Fluorescence Biosensors) Downloadkeyboard_arrow_down Browse Figures Versions Notes Abstract A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 μm edge length and 100 μm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 μM. Proof-of-concept measurements of streptavidin conjugate Qdot? 605 and Amino PEG Qdot? 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement. Keywords: Point-of-Care; multiplex; microLED array; SPAD; fluorescence; lifetime fluorescence; GaN; CMOS; microLED driver 1. Introduction #perioperative_care #caretaker_medical #continuous_cardiac_output #true_invasive_blood_pressure